Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Mar;178(3):1245–1256. doi: 10.1016/j.ajpath.2010.11.070

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.

PMC Copyright notice

IL-8 mRNA and protein expression is up-regulated by PGE₂ and hypoxic conditions (0.5% O₂) in endometrial epithelial cells. A: An endometrial epithelial cell line (EP2S cells) treated with 100 nmol/L of PGE₂ under normoxic conditions (21% O₂) showed significantly increased levels of IL-8 mRNA at 4 and 8 hours, compared with cells treated with vehicle (V) at the same time point (n = 3). B: EP2S cells under hypoxic conditions showed a slower pattern of IL-8 mRNA induction, reaching significance after 8 hours (n = 3). C: EP2S cells treated simultaneously with PGE₂ and hypoxia revealed a synergistic increase in IL-8 mRNA expression after 24 hours when compared with treatment with PGE₂ in normoxia or hypoxia alone (n = 3). D: This synergistic increase was also observed when examining IL-8 secreted protein levels in EP2S-conditioned medium from the same experiments (n = 3). *P < 0.05. **P < 0.01.