Table 1.
Cell cycle distribution of WI38 fibroblasts following Ku80 depletion
| 24 hours after infection | Control | Ku-depleted |
| G1 | 61.1% | 61.4% |
| S | 15.1% | 5.7% |
| G2/M | 23.8% | 32.9% |
| 48 hours after infection | Control | Ku-depleted |
| G1 | 64.5% | 58.6% |
| S | 13.7% | 8.5% |
| G2/M | 21.8% | 32.9% |
WI38 cells were infected with Ku80 shRNA for 24 hours then collected for cell cycle analysis either another 24 or 48 hours after infection was complete. Cells were stained with the Guava Cell Cycle reagent and analyzed on the Guava EasyCyte Mini flow cytometer for DNA content. The data are presented as the percentage of cells within either G1, S, or G2/M phases of the cell cycle, with cell debris or apoptotic cells not included.