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. 2010 Dec 15;9(24):4841–4847. doi: 10.4161/cc.9.24.14093

Figure 2.

Figure 2

NFκB/p65 mediates the reduction of ΔNp63α levels by cisplatin. (A and B) JHU-022 and p53/p63 deficient H1299 cells were transfected with increasing concentrations (0, 0.5, 1 and 1.5 µg) of NFκB/p65 expression plasmids as indicated; western blot was performed using the indicated antibodies. p53/p63 deficient H1299 cells were also transfected with 1 µg of ΔNp63α expression plasmid. (C) JHU-022 cells were transfected with increasing concentrations of either NFκB/p65 RNAi plasmid and subjected to western blot analysis using anti-p63 antibodies to assess the endogenous levels of p63. (D) JHU-022 cells were transfected with or without NFκB/p65 plasmid 24 h after transfection, the cells were treated with or without 75 µM cisplatin for 8 h, and cell lysates were subjected to western blot using anti-p63 antibodies.