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. 2010 Dec 15;9(24):4841–4847. doi: 10.4161/cc.9.24.14093

Figure 4.

Figure 4

NFκB/p65 counteracts the repressive transcriptional activity of ΔNp63α. (A) JHU-022 cells were transfected with either p21 or Bax luciferase promoter construct along with Renilla luciferase plasmid, with or without NFκB/p65 or NFκB/p65Δ450 and/or ΔNp63α, as indicated. The amount of DNA per transfection was kept constant by using empty pCDNA3.1 vector. At 24 h post-transfection, the luciferase activity was determined. The transfection efficiency was standardized against Renilla luciferase. Results shown are representative of three independent experiments. *indicated p ≤ 0.001. (B) JHU-022 cells were transfected with increasing concentrations of NFκB/p65 expression plasmid and western blot analysis was performed with the indicated antibodies to assess the endogenous levels of the indicated proteins. (C) (i) JHU-022 cells were transfected with empty vector or expression vector encoding NFκB/p65Δ450, and western blot analysis was performed with the indicated antibodies to assess the endogenous levels of the indicated proteins. (ii) A diagramatic representation of the NFκB/p65 domains.