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. 2010 Dec 15;9(24):4884–4892. doi: 10.4161/cc.9.24.14156

Figure 2.

Figure 2

BAFF-R-expressing early B cell lines show significant BAFF-R promoter reporter activity. The genomic region spanning the 0.5 kb upstream of TNFRSF13C, the gene encoding BAFF-R, ending 6 bp 5′ of the start codon, was cloned and inserted into firefly luciferase reporter vector pGL3-Basic. This reporter was co-transfected into the target cell lines by electroporation with a fixed amount of control Renilla luciferase reporter vector pRL-TK. Specific promoter activity is reported as relative light units, the ratio of firefly: Renilla luciferase activity normalized to the relative activity of the empty pGL3-Basic vector. **p < 0.01.