Figure 5.
The regulation of BAFF-R expression in CLL is primarily post-transcriptional. (A) Relative expression of BAFF-R and NFκB family member c-Rel in CLL and normal peripheral blood B cells. Expression levels were determined by qRT-PCR and normalized to 18S rRNA. (B) The relationship between c-Rel and BAFF-R mRNA levels in CLL and normal B cells. (C) Immunoblot for BAFF-R and β-actin in five CLL and four normal peripheral blood B cell samples. Densitometric analysis was performed and quantified as a ratio of adjusted intensity volume of BAFF-R to β-actin and normalized to a scale of 0 to 10. Relative values are noted above the BAFF-R blot.