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Cdk4−/− cells proliferate, secrete cytokines in response to stimulation. (A) Thymocytes were incubated for 48 h with IL-2. (B) Splenocytes were incubated for 48 h on anti-CD3 antibodies. Proliferation was measured by BrdU incorporation. (C) Cytokine expression in conditioned media from stimulated splenocytes. N = 3/group. Means ± SD are shown.
