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. 2010 Dec 1;9(23):4656–4665. doi: 10.4161/cc.9.23.13974

Figure 1.

Figure 1

Loss of Akap12 leads to multinucleation, polyploidy and premature senescence. (A) Phase contrast images of Akap12+/+, +/− and −/− (WT, het and KO) MEF (upper). Scale bar, 30 µm. Thick arrows, binucleated cells; thin arrows, stress fiber bundles. Lower: Immunoblotting of WT, het and KO MEF lysates probed for Akap12 (showing α and β isoforms) or Gapdh. (B) Percentage of multinucleated cells in WT- vs. KO-MEF (left) versus the number of the nuclei/cell (right). *p < 0.001. (C) Cell proliferation rates in P3 vs. P8 WT- and KO-MEF. (D) Left: P6 KO-MEF display premature senescence based on Saβgal staining and enlarged cell morphologies. Scale bar, 30 µm. Right: percentage of Saβgal-stained cells from P6. *p < 0.001.