Figure 3.

Inhibition of Cot1 expression by Cot1 siRNA enhances SIL/1,25D-induced G₁ cell cycle arrest in AML cells. HL60, U937 and THP1 cells were transfected with Cot1 siRNA (siCot1) or scrambled control siRNA (siControl) oligonucleotides. Following 48 h, the cells were exposed to the indicated agents for an additional 48h. Cell cycle distribution was determined by flow cytometry of propidium iodide (PI)-stained cells, and the G₁ to S phase ratios were calculated to asses the G₁ block. Asterisk *indicates, p < 0.05 for the comparison of cells transfected with siCot1 RNA and exposed to SIL/1,25D vs. corresponding SIL/1,25D samples transfected with siControl RNA (n = 3).