a. Western blotting reveals endogenous levels of angiogenin produced by both M17 and SH-SY5Y dopaminergic cell lines. Tubulin was used as loading control.
b. A robust increase in intracellular angiogenin staining is observed after treatment with exogenous angiogenin. SH-SY5Y cells were immunostained using a goat polyclonal antibody against angiogenin and a cy3-conjugated donkey anti-goat antibody at A) control, B) 2 hours, C) 12 hours, and D) 36 hours after treatment with 100nM recombinant angiogenin. Scale bar represents 25μm.