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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Neurochem. 2010 Dec 22;116(3):334–341. doi: 10.1111/j.1471-4159.2010.07112.x

Figure 3. Angiogenin induces Akt phosphorylation at serine 473.

Figure 3

a. Representative Western blot demonstrates increased Akt phosphorylation at serine 473 within 30 seconds following treatment of SH-SY5Y cells with recombinant angiogenin (100nM). This Akt phosphorylation is maintained for several hours. Total Akt levels are unchanged with angiogenin treatment. Actin is used as a loading control.

b. Densitometric quantification of Western blots against phosphorylated Akt. Results reflect three independent experiments. **p<0.01 (One-way ANOVA with Tukey’s post-hoc test). Error bars reflect SEM.