Skip to main content
. 2011 Jan 1;10(1):73–81. doi: 10.4161/cc.10.1.14243

Figure 2.

Figure 2

The NPF motifs of PACSIN 2 are required for cyclin D1 binding. (A) Schematic representation of PACSIN 1, 2 and 3. (B) The mammalian expression vectors encoding PACSIN 1, 2 or 3 were co-expressed with FLAG-tagged cyclin D1 and subsequent IP-WB was conducted. The Myc epitope of PACSINs are shown by western blot of cellular lysates. Co-precipitation of cyclin D1 with PACSIN 2 is shown by immunoprecipitation of cyclin D1 with FLAG antibody. (C and D) HEK293T cells were co-transfected with mutant constructs of PACSIN 2 (as indicated) together with vector encoding FLAG-cyclin D1. Immunoprecipitation was performed with an anti-Myc antibody following by western blot using FLAG antibody to detect cyclin D1.