Figure 5.

PACSIN 2 regulation of cell spreading depends on cyclin D1 expression. (A) Cyclin D1^−/− cells transduced with either cyclin D1a or cyclin D1b retrovirus and transfected with either control or siPACSIN 2 were assessed for cell spreading at different time points after seeding. (B) Quantitative analysis of cell spreading (arbitrary unit). Student t-test was used for quantitative analysis of cell spreading. p-value and number of cells analyzed were indicated in the figure. N is for the number of cells counted. Data are representative of three separate experiments.