Table 1.
Lifespan, cycle time, relative growth rate per generation in selected cell size mutants
| Size1 | ||||||
| Strain2 | Start | End | Cycle time3 | LS4 | % Change5 | RGR6 |
| whi5Δ | 5.7 | 9.1 | 108.6 | 34.7 | 31% | 0.6 |
| fob1Δ | 5.9 | 9.83 | 91.3 | 32.4 | 22% | 0.7 |
| ssf1Δ | 6.4 | 12.3 | 153.4 | 44.1 | 66% | 0.8 |
| cln3Δ7 small | 6.5 | 10.5 | 99.4 | 18.3 | −31% | 1.3 |
| rpl42aΔ | 6.7 | 11.5 | 131.6 | 40.4 | 52% | 0.7 |
| whi5Δ7 Large | 6.9 | 10.0 | ND | 27.3 | 3% | 0.7 |
| Wild type8 | 6.9 | 11.3 | 83.6 | 26.5 | - | 1.0 |
| rpl42bΔ | 6.9 | 9.7 | 107.5 | 9.7 | −64% | 1.7 |
| ssf1Δ cdc289 | 7.4 | 9.7 | 137.3 | 12.9 | −51% | 1.1 |
| Wild type8,10 | 7.5 | 11.1 | ND | 16.8 | −37% | 1.3 |
| cln3Δ whi5Δ11 | 7.6 | 12.5 | 83.9 | 20.3 | −24% | 1.5 |
| Wild type7 Large | 7.8 | 11.0 | ND | 20.9 | −21% | 0.9 |
| bck2Δ | 7.8 | 11.9 | 88.1 | 20.0 | −25% | 1.2 |
| ssf1Δ7 Large | 7.9 | 11.7 | ND | 31.6 | 19% | 0.7 |
| hxk2Δ12 | 8.0 | 12.2 | 154.2 | 37.8 | 43% | 0.7 |
| ssf1Δ nocodazole13 | 8.1 | 10.3 | 88.3 | 7.1 | −73% | 1.8 |
| cln3Δ | 8.2 | 12.0 | 125.1 | 15.4 | −42% | 1.5 |
| ssf1Δ9 Large II | 8.8 | 11.9 | 114.6 | 19.0 | −28% | 1.0 |
| Wild type7 Large II | 9.0 | 10.9 | 73.1 | 9.7 | −63% | 1.2 |
| whi5Δ7 Large II | 9.0 | 12.3 | ND | 11.1 | −58% | 1.8 |
| cln1Δ cln2Δ14 | 9.1 | 13.2 | 89.6 | 8.8 | −67% | 2.8 |
| apn1Δ | 9.2 | 11.8 | 176.4 | 10.9 | −59% | 1.4 |
| cln3Δ7, Large | 9.5 | 13.7 | 90.5 | 7.6 | −71% | 3.3 |
| cln3Δ7 Large II | 11.1 | 12.5 | 95.6 | 2.8 | −89% | 3.1 |
The cell diameter (microns) of all virgin daughter cells was determined at the start and end of the replicative lifespan assays. Numbers are the average for all cells. Due to technical complications, start sizes are not birth sizes but are closer to the size at which virgin daughters bud.
All strains are homozygous deletions in wild type BY4743 background. Specific strain genotypes, experimental conditions, or noted results are listed in superscripts. All cells were propagated on 2% glucose at all times.
Cycle time in minutes was determined by calculating the time between three subsequent buddings. Experiments were conducted using 30–50 individual virgin daughters for each sample. ND: not determined.
LS is average lifespan of each experiment.
Percent change in lifespan of each experiment compared to wild type cells.
RGR is relative growth rate per generation calculated by dividing net growth (size at end of the experiment minus size at start) by the average lifespan and normalized to wild type (set to 1).
Centrifugal elutriation was used to size-fractionate log phase YPD cultures.
WT is diploid wild type (BY4743).
An ssf1Δcdc28ts was created by mating a MATα ssf1Δ strain with a MATa strain carrying cdc28-17ts mutations in the s288c background. Heterozygotes were sporulated. Haploids with an ssf1Δcdc28ts genotype were determined by PCR and cell cycle arrests at the restrictive temperature. Subsequently, homozygous diploids were generated by mating.
The selection of virgin granddaughters and great-granddaughters from huge wild type cells resulted in smaller longer-lived cells further demonstrates that lifespan correlates with size.
cln3Δ::LEU2 whi5Δ::NAT.
The start size of these deletions was unexpectedly large despite measurements in other experiments or procedures that reproducibly demonstrated that hxk2Δ virgin daughters are small.
ssf1Δ cells were treated with nocodazole as described to produce large cells within a single generation.
cln1Δ::LEU2 cln2Δ::KANMX.