Figure 3.

Simulation of the relative importance of growth rate and replicative potential on fitness of yeast cells after 40 wild type cell division cycles. (A) The replicative life span of a mutant has relatively little effect on fitness, except in cases where life span is reduced to less than about 3 generations. Replicative life span for wild type is set at 26 generations. Doubling time for mutant and wild type is defined as equal (90 minutes). (B) Doubling time of the mutant has a relatively large effect on relative fitness. Wild type doubling time is set at 90 minutes. Replicative life span for mutant and wild type is defined as equal (26 generations). (C) Comparison of the in silico predicted relative fitness for dbp3Δ and sch9Δ cells based on experimentally measured replicative life span and doubling time values with the experimentally measured relative fitness of these mutants. Comparisons of the remaining mutants to in silico calculations can be found in Supplemental Figure 1.