Figure 4.

Reduced vitality of long-lived mutants occurs early in life. (A) The median number of daughters produced per mother cell over the first three time-points in a standard replicative life span assay is shown for experimentally-matched wild type (BY4742) and long-lived mutants (pooled data for 32 strains showing a significant defect in relative fitness). Each time point corresponds to removal of daughter cells by microdissection after 90–150 minutes of incubation at 30°C. *p < 0.05, **p < 0.01. (B) Cumulative daughter cell production per mother cell derived from a replicative lifespan experiment. Experimental time points are the same as described in (A).