Table 1.
Summary of data from tsetse fly transmission of J10 transfected clones
Cell line* | Midguts† | SGs (pair)† | |||
Total infected | Number with fluorescent cells | Total infected | Number with fluorescent cells | Days after infection‡ | |
J10 YFP::DMC1 clones 1 and 2 | 349/499 (70%) | 0/124 (0%) | 15/349 (4%) | 14/15 (93%) | 14–31 |
J10 YFP::MND1 clones 1 and 2 | 219/340 (64%) | 0/110 (0%) | 9/219 (4%) | 9/9 (100%) | 17–33 |
J10 YFP::HOP1 clones 1 and 2 | 307/339 (91%) | 0/74 (0%) | 44/307 (14%) | 35/44 (80%) | 20–28 |
J10 YFP::SPO11 clones 1, 2, and 3 | 456/727 (63%) | 0/66 (0%) | 17/456 (4%) | 0/17 (0%) | NA |
*At least two different clones were analyzed for each fusion construct.
†The infection rates for midguts and SGs are similar to those routinely achieved for WT J10, showing that the fusion constructs did not have an adverse effect.
‡Fluorescent trypanosomes were observed in the SGs during the time window given.