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. 2011 Feb 14;108(9):3725–3730. doi: 10.1073/pnas.1100446108

Fig. 4.

Fig. 4.

E-selectin is up-regulated by endothelial FAK activation in hyperpermeable areas and mediates metastatic cancer cell homing in vivo. (A) E-selectin expression in areas of high vs. low EB leakage in wild-type mice stimulated with i.v. ETCM (as measured by immunohistochemistry). *P < 0.05, n = 4. (B) E-selectin expression in wild-type vs. tTA-FRNK mice stimulated with i.v. ETCM. *P < 0.05, n = 4. (C) In vivo assay of tumor cell homing to ETCM-stimulated lungs of wild-type and tTA-FRNK mice, with or without administration of an E-selectin blocking antibody. *P < 0.05, n = 3–4. (D) Number of metastatic cancer cells homing to areas of high vs. low EB leakage in the lungs of wild-type and E-selectin−/− mice 5 h after stimulation with i.v. ETCM. *P < 0.05, n = 4. (E) Metastatic cancer cell homing to the lungs of age- and sex-matched C57BL/6 (wild-type) and E-selectin−/− mice 24 h after stimulation with i.v. ETCM. *P < 0.05, n = 4. (F) Metastatic cancer cell homing to the lungs of tumor-bearing wild-type and E-selectin−/− mice measured 48 h after TCM and tumor cell injection. *P < 0.05, n = 4. Error bars are SEM.