Fig. 3.

Specific recognition of peptide mimotopes enables alloreactivity. (A) Four nonalloreactive T-cell hybrids with distinct TCR were examined for the specificity of recognition of mimotopes enabling alloreactivity. (B) Specificity of responses to I-E^k mimotopes was assessed by using peptide pools with defined I-E^k anchor residues and limited amino acid pools at a single TCR contact residue combined with unlimited amino acid use at other TCR contacts. Responses were assessed by ELISA measuring IL-2 production after 24-h culture of T-cell hybrids with CHO-E^k cells and 100 μM peptide pools. Results shown are mean ± SEM of one of three independent experiments. (C) T-cell hybrid recognition of limited peptide mimotope pools defined as IL-2 production in response to stimulation with CHO-E^k and 100 μM peptide pools with background IL-2 production from unstimulated T-cell hybrid cultures subtracted. Data shown are means of three independent experiments. (D) Dose–responses of T-cell hybrids to mimotopes assessed by measurement of IL-2 production from 24-h culture of T-cell hybrids with CHO-E^k. Data are mean ± SEM from one of three independent experiments.