Skip to main content
. 2011 Feb 14;108(9):3695–3700. doi: 10.1073/pnas.1017015108

Fig. 4.

Fig. 4.

Recognition of different allogeneic MHC requires distinct peptide recognition. (A) Response to I-Ep peptide mimotope pools was assessed by measuring IL-2 production by ELISA after 24-h culture of T-cell hybrids with CHO-Ep cells and 100 μM peptide pools. Responses to limited mimotope pools were defined as IL-2 production in response to stimulation with CHO-Ep and 100 μM peptide pools with background IL-2 production from unstimulated T-cell hybrid cultures subtracted. Data shown are means of three independent experiments. (B) Dose–response of 1F2 T-cell hybrid to I-Ep single peptide mimotopes was measured by ELISA of 24-h culture supernatants from T-cell hybrid cells cultured with CHO-Ep plus single peptide mimotopes. Data are mean ± SEM from one of three independent experiments. (C) IL-2 production of 1F2 hybrid cells cultured 24 h with CHO-Ek or CHO-Ep in the presence of 30 μM of single peptide mimotopes. Data are mean plus SEM from one of three independent experiments.