Fig. 3.

SBS binding activates SlmA. (A) Sedimentation assays were performed as in Fig. 1 A–B, except lower levels (250 nM) of SlmA were used and the reactions also contained 30 bp of WT or mutant SBS17 dsDNA fragments (50 nM) as indicated. (B and C) FtsZ polymers were formed in reactions containing FtsZ (2 μM) and GTP (2 mM) without (B) or with (C) the addition of SlmA (2 μM) and 30 bp of SBS17 dsDNA (400 nM). Reactions were spotted onto carbon-coated grids 5 min after GTP addition, negatively stained with uranyl formate, and visualized by electron microscopy. (Scale bar, 100 nm.)