Fig. 5.

A low concentration of rapamycin that is subthreshold for WT mice impairs LTM reconsolidation in mTOR^+/− mice. (A) Scheme of the experimental protocol. (B) Animals were trained as described in Fig. 4. Two days after training, memory was reactivated, and WT and mTOR^+/− mice were administered either vehicle or rapamycin (40 mg/kg) immediately after reactivation. Contextual fear conditioning was determined by measuring the time spent freezing (as pecentage of total time) before the conditioning (Naïve, during a 2-min period), during reactivation (during a 5-min period), 2 h (PR-STM, during a 5-min period) and 22 h (PR-LTM, during a 5-min period) after reactivation.