Fig. 1.

Effect of NF-κB pathway inhibitors and activator on AAV vector–mediated EGFP expression in HeLa cells in vitro. Cells were pretreated with various concentrations of inhibitors and activators for 12 h and transduced with 2 × 10³ AAV-EGFP vg per cell. (A) Transgene expression was detected by fluorescence microscopy 48 h postinfection. (B) Quantitative analyses of the data from A. Images from five visual fields were analyzed as described in Materials and Methods. *P < 0.001. (C) Western blot analysis of HeLa cell extracts transduced with scAAV vectors in the presence of NF-κB modulators. The samples were analyzed by using anti-p65 and anti-IκB antibodies (classical pathway) and anti-p100/p52 antibody (alternative pathway) for detection of NF-κB signaling. These results are representative of two independent experiments.