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AAV-EGFP vector-mediated transduction of primary human monocyte-derived dendritic cells in the presence of NF-κB modulators. (A) Transgene expression was detected by flow cytometry 48 h posttransduction. (B) Western blot analysis for NF-κB activation in nuclear extracts from DCs, mock-transduced or transduced with 2,000 vg/cell of scAAV vectors ± NF-κB modulators. I-DC, immature dendritic cells; DC, dendritic cells; M-DC, mature dendritic cells.
