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. 2011 Feb 8;108(9):3689–3694. doi: 10.1073/pnas.1015406108

Fig. 3.

Fig. 3.

E6446 prevents priming of TLR proinflammatory responses. C57BL/6 mice were treated with 60 mg·kg−1·d−1 of E6446 or vehicle 1 d before and during 6 d postinfection with 105 P. chabaudi iRBCs. (A) At 7 d postinfection, spleens were harvested and cultured in the presence of LPS (1 μg/mL), Pam3cysK4 (1 μg/mL), Poly:IC (100 μg/mL), CL075 (100 ng/mL), CpG ODN (1 μg/mL), or P. chabaudi extract (100 μg/mL) for 48 h. Cytokine levels in cell culture supernatant or in sera were assessed by ELISA. P.c., P. chabaudi. (B) Mice were treated with 120 mg·kg−1·d−1 during 6 d postinfection with 105 P. chabaudi iRBCs. Mice were challenged i.v. at 7 d postinfection with 10 μg of LPS. Survival was monitored from 12–48 h after LPS challenge. An asterisk marks where comparisons reached statistically significant (P < 0.05).