Fig. 4.

E6446 protects mice against PbA-mediated CM. C57BL/6 mice were treated with 120 mg·kg⁻¹·d⁻¹ of E6446 1 d before and during 12 d postinfection with 10⁵ PbA-infected erythrocytes (iRBCs). Mice treated with vehicle (acidified water) were infected and used as a control group. (A) Survival and parasitemia were compared between groups of mice at various days postinfection. (B) Effect of emergency treatment with E6446 on ECM. Mice were infected with 10⁵ PbA iRBCs and treated with E6446 beginning at different days postinfection. p.i., postinfection. Survival was monitored throughout infection. (C) Cytokine levels were assessed in the sera (10 mice per group) or in supernatants of splenocytes cultured in the presence of LPS (1 μg/mL) or CpG ODN (1 μg/mL). (D) Analysis of intracellular staining of cytokines in CD11c⁺ MHCII⁺ splenic DCs from vehicle- or E6446-treated mice at 6 d postinfection. Results are from a sum of four different experiments that yielded similar results. An unpaired two-tailed Student's t test or Mann–Whitney U test was used to compare means between cytokine levels detected in the vehicle-treated or E6446-treated group of mice. A P value <0.05 was considered to be statistically significant.