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. 2011 Mar 3;6(3):e17542. doi: 10.1371/journal.pone.0017542

Figure 5. Immunoprecipitation assays of transfected HFE splice variants.

Figure 5

HepG2 cells were transfected with pEGFP_HFE_full length, pEGFP_HFE_C282Y, pEGFP_HFE_skip2 or pEGFP_HFE_ivs4 constructs. (A) Protein expression in HepG2 transfectants prior to immunoprecipitation. (B) Cell lysates and (C) cell media were subjected to immunoprecipitation using a mouse anti-GFP monoclonal antibody and G-agarose beads. Blots were incubated with anti-TfR1, -GFP and -β2M antibodies for protein detection. The predicted molecular mass of the proteins is indicated in kDa.