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Susceptibility of carcinoembryonic antigen (CEA)-positive MKN45 and CEA-negative MKN74 cells to wild-type gD herpes simplex virus type 1 and receptor-restricted derivatives. Cells were infected with K26GFP, nectin-1-restricted K-d5-28V, or HVEM-restricted K-222/3NI at 100 genome copies/cell and stained at 8 hours postinfection with anti-VP16 antibody and Cy3-conjugated secondary antibody. The lower panels for each cell line show phase-contrast images of the same fields.
