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Transient immune response to canine factor VIII (cFVIII) observed in L51 after administration of adeno-associated virus 8 (AAV8)-cFVIII. (a) Anti-cFVIII IgG2 antibodies were detected by a cFVIII-specific IgG2 enzyme-linked immunosorbent assay. No IgG1 antibodies were detected. Bethesda assay detects FVIII neutralizing antibodies. (b) Canine FVIII activity was determined by Coatest assay.
