Figure 6.

Liver-specific insulin gene expression in rAd-SP23137-rINSfur-treated NOD.scid mice. rAd-SP23137-rINSfur (10¹⁰ VP) viruses were administered into STZ-induced diabetic NOD.scid mice. After the treated animals showed normal glucose levels at 2 weeks postinfection, they were sacrificed and the various organs were removed. (a) Immunohistochemical staining for insulin in the liver. The stained cells are indicated as arrows. (b) RT-PCR. Total RNAs were isolated and RT-PCR was performed using primers for rat insulin or HPRT. Ht, heart; Kd, kidney; Li, liver; Lu, lung; RT-PCR, reverse transcriptase-PCR; Sp, spleen; STZ, streptozotocin; VP, viral particles.