Skip to main content
. 2010 Nov 30;19(3):584–593. doi: 10.1038/mt.2010.269

Figure 3.

Figure 3

Expression of pluripotency and differentiation genes by reverse transcriptase-PCR. (a) Total RNA from undifferentiated induced pluripotent stem cells (iPSCs) was extracted and analyzed by reverse transcriptase-PCR for the expression of the pluripotency genes octamer-binding transcription factor 4 (OCT4), sex determining region Y-box 2 (SOX2), cytoplasmic Myc (c-MYC), NANOG, teratocarcinoma-derived growth factor 1 (TDGF1), and REX1. CB CD34+ human hematopoietic stem cells (HSCs) were used as a control to detect expression in the “starter cells.” H9 human embryonic stem cells (hESCs) were used as a pluripotency positive control. (b) Total RNA was extracted from differentiated (D) cells from the cystic bodies which formed in the iPSC/OP9 cocultures and analyzed by RT-PCR for the expression of genes from all three germ layers including α-fetoprotein (AFP), cytokeratin 8 (CK8), brachyury (BRACHY), Msh homeobox 1 (MSX1), and paired box gene 6 (PAX6). Undifferentiated (U) cells were used as negative controls for expression. Undifferentiated and differentiated H9 hESCs were used as negative and positive controls, respectively. GAPDH was used as an internal loading control. Experiments were performed in duplicate. CB, cord blood; EGFP, enhanced green fluorescent protein; WT, wild type.