Figure 7.

HIV-1 challenge of induced pluripotent stem cells (iPSCs) derived macrophages. Macrophages derived from the wild type (WT) (diamonds), enhanced green fluorescent protein (EGFP) (squares), and anti-HIV (triangles) iPSCs were challenged with (a) an R5-tropic BaL-1 or (b) a dual-tropic 89.6 strain of HIV-1 at an multiplicity of infection of 0.05. On various days postinfection, cell culture supernatants were sampled and analyzed for p24 antigen by enzyme-linked immunosorbent assay. Experiments were performed in triplicate. (c) On day 21 postinfection, total RNA from uninfected (UI) and HIV-1 challenged macrophages were analyzed by reverse transcriptase-PCR for the expression of spliced (tat) and unspliced (pol) HIV-1 transcripts.