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The effect of the polypurine tract (PPT) deletion on transgene expression from lentiviral vectors in vivo. Green fluorescent protein expression mediated by PPT+ (vTK945) or PPT− (vTK1023) vectors, packaged into particles containing either functional (IN+) (upper) or deficient (IN−) (lower) integrase, in the striatum of the rat brain, was measured 2 months after vector infusion. The indicated scale bar is equal to 20 µm.
