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. 2010 Dec 21;19(3):557–566. doi: 10.1038/mt.2010.278

Figure 2.

Figure 2

Effect of concentrated lentiviral vector on the growth and infectivity of C8166 cells. (a) The effect on concentrated vector was assessed in the nonadherent C8166 cell line and the adherent MRC-5 cell line. Cells were exposed to concentrated CSCGW vector for 4 hours and cell number counted after 48 hours. Concentrated vector (~5,000 ng/ml) was used undiluted or diluted 1:10 and 1:100. Camptothecin was used as a positive toxin control. The data represents the average of three cultures ± SD. (b) The effect of concentrated vector on the growth of C8166 cells was measured at three concentration, 1,000, 5,000, and 10,000 ng/ml of p24 using the CSCGW vector. Duplicate cultures were exposed to vector preparations on day 0 and the total number of cells was determined in each culture after 5 or 6 days of growth (experiment 1 or 2, respectively). Data represents the average of two cultures ± SD for two independent experiments. (c) The inhibitory effect of concentrated vector on C8166 cell infectivity was measured by introducing increasing amounts of a null vector (CSO) into a lentiviral vector preparation of the green fluorescent protein (GFP)-expressing CSCGW vector. The x-axis represents the amount of CSO vector as measured by p24 ELISA. The y-axis represents the GFP-expressing cells as a percentage of control (no CSO vector). The percentage of control cells expressing GFP ranged between 13.5 and 20.1%. The data represents the average of three independent experiment ± SD.