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. 2011 Feb 8;104(5):769–780. doi: 10.1038/bjc.2011.21

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Copyright © 2011 Cancer Research UK

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AZD1152-HQPA-dependent modulation of cell pathways. (A) MiaPaCa-2 cells were incubated with AZD1152-HQPA for 1 day, followed by 0 or 3 days drug wash out, and then histone 3 phosphorylation was analysed by immunofluorescence staining (Ser-10 pH3 staining in red and DAPI in blue) and western blotting. (B and C) Cells were incubated with AZD1152-HQPA and protein extracts were analysed by western blotting. The amount of p-AKT and p53 was determined using β-actin to normalise values.