Table 1. Reproducibility of the qrt-PCR assay based on pLSUO plasmid standard curve.

Copy number	Mean Ct ± SDa	CVCt%b	CVCn%c
106	12.88±0.06	0.4	4.0
105	16.89±0.61	3.6	33
104	20.21±0.41	2.0	26
103	23.68±0.38	1.6	23
102	27.15±0.38	1.4	23
101	30.89±0.49	1.6	33
5	31.36±0.23	0.7	15
2	32.09±0.71	2.2	46

^aMean Ct values measured in 8 independent experiments ± standard deviation (SD).

^bCoefficient of variation of cycle threshold.

^cCoefficient of variation of copy number.