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. 2011 Mar 3;6(3):e17699. doi: 10.1371/journal.pone.0017699

Table 5. Qrt-PCR assay and microscopy analysis of Ostreopsis cf. ovata cell number from surface seawater samples collected in 2009 at Conero Riviera (northern Adriatic Sea) and calculation of LSU gene copy number per cell by qrt-PCR.

Sample no. Sampling period Abundancea(cells l−1 ± SD) LSU copy no.c(cell−1 ± SD)
qrt-PCR Microscopy
23 March 25th n.d.b n.d. n.d.
24 April 7th n.d. n.d. n.d.
25 May 7th n.d. n.d. n.d.
26 May 18th n.d. n.d. n.d.
27 June 3rd n.d. n.d. n.d.
28 June 15th n.d. n.d. n.d.
29 July 3rd n.d. n.d. n.d.
30 July 17th n.d. n.d. n.d.
31 July 31st n.d. n.d. n.d.
32 August 6th n.d. n.d. n.d.
33 August 11st n.d. n.d. n.d.
34 August 21st n.d. n.d. n.d.
35 August 27th 11±3 160±131 1142±74
36 September 3rd 125924±5100 92480±3699 971±39
37 September 9th 100±50 360±241 1080±48
38 September 15th 250±75 840±370 1058±65
39 September 23rd 16625±775 15200±1216 1029±58
40 September 29th 15675±2700 13750±2062 1013±52
41 October 6th 2475±75 680±313 1030±40
42 October 21st n.d. 20±40 n.d.
43 October 28th n.d. n.d. n.d.
a

Mean abundance measured in triplicates ± standard deviation (SD).

b

Not detected.

c

Mean LSU copy number measured in triplicates ± standard deviation (SD).