Table 5. Qrt-PCR assay and microscopy analysis of Ostreopsis cf. ovata cell number from surface seawater samples collected in 2009 at Conero Riviera (northern Adriatic Sea) and calculation of LSU gene copy number per cell by qrt-PCR.
Sample no. | Sampling period | Abundancea(cells l−1 ± SD) | LSU copy no.c(cell−1 ± SD) | |
qrt-PCR | Microscopy | |||
23 | March 25th | n.d.b | n.d. | n.d. |
24 | April 7th | n.d. | n.d. | n.d. |
25 | May 7th | n.d. | n.d. | n.d. |
26 | May 18th | n.d. | n.d. | n.d. |
27 | June 3rd | n.d. | n.d. | n.d. |
28 | June 15th | n.d. | n.d. | n.d. |
29 | July 3rd | n.d. | n.d. | n.d. |
30 | July 17th | n.d. | n.d. | n.d. |
31 | July 31st | n.d. | n.d. | n.d. |
32 | August 6th | n.d. | n.d. | n.d. |
33 | August 11st | n.d. | n.d. | n.d. |
34 | August 21st | n.d. | n.d. | n.d. |
35 | August 27th | 11±3 | 160±131 | 1142±74 |
36 | September 3rd | 125924±5100 | 92480±3699 | 971±39 |
37 | September 9th | 100±50 | 360±241 | 1080±48 |
38 | September 15th | 250±75 | 840±370 | 1058±65 |
39 | September 23rd | 16625±775 | 15200±1216 | 1029±58 |
40 | September 29th | 15675±2700 | 13750±2062 | 1013±52 |
41 | October 6th | 2475±75 | 680±313 | 1030±40 |
42 | October 21st | n.d. | 20±40 | n.d. |
43 | October 28th | n.d. | n.d. | n.d. |
Mean abundance measured in triplicates ± standard deviation (SD).
Not detected.
Mean LSU copy number measured in triplicates ± standard deviation (SD).