Figure 1. Relative abundance of L. pneumophila genes differentially expressed during growth in broth and during intracellular growth in A. castellanii.

Colors correspond to categories of genes in the COG database. Exponential (E) and post-exponential (PE) growth denotes L. pneumophila Philadelphia-1 grown in BYE broth. Replicative phase (RP) and transmissive phase (TP) represents L. pneumophila Philadelphia-1 growth inside of A. castellanii Neff. In the late growth stages genes coding for proteins important for replication and energy production are replaced by genes coding for proteins involved in cell motility and signal transduction. Additionally in the transmissive phase genes coding for proteins important for lipid transport and metabolism are also strongly up-regulated. The relative gene abundance is listed in Table S4.