Figure 2. Phenotypic and RNA blot analyses of primary OsMT2b-RNAi transgenic lines.

A. RNA blot analysis of the endogenous OsMT2b transcript. Twenty µg of total RNAs isolated from two independent pOsMT2b::OsMT2b-RNAi transgenic lines (1 and 2) and the control line (Ctrl) were separated on denaturing agarose gel, stained with ethidium bromide, transferred to a nylon membrane, hybridized with α ³²P-labeled OsMT2b cDNA fragment, and analyzed by autoradiography (upper panel). The lower panel shows the ethidium bromide-stained ribosomal RNAs used as a loading control. B. Comparison of the seed embryo between a representative OsMT2b-RNAi transgenic line (1) and the control (Ctrl). Scale Bar = 1 mm. C. Phenotypic comparison of panicles/spikelets between the representative pOsMT2b::OsMT2b-RNAi transgenic line (1) and the control (Ctrl).