Skip to main content
. 2011 Mar 3;6(3):e17444. doi: 10.1371/journal.pone.0017444

Table 1. Sequences of primers.

Names of primers abbreviation sequence (5′to 3′) Description
OsMT2b promoter f P-MT-F aaaaaagcttgagatgctaatcaagtctctctg Hind III
OsMT2b promoter r P-MT-R aaaagatatcagatgttgttgctgattgagctc EcoR V
OsSRT1 promoter f P-SRT-F aaaagaattcgtgcttgtgtgtcattctaccc EcoR I
OsSRT1 promoter r P-SRT-R aaaaggtaccggacatggtggttcagttgaaccc Kpn I
OsPDK1 promoter f P-PDK1-F aaaagaattcgtagtgtcaggctgtcagcaac EcoR I
OsPDK1 promoter r P-PDK1-R aaaatctagaccctaccgacaacagcaccac Xba I
OsPDK2 promoter f P-PDK2-F aaaagaattccgctgtactatgagtcgtacc EcoR I
OsPDK2 promoter r P-PDK2-R aaaaggtaccatcatgtagcgcaggctcac Kpn I
Ubi promoter f P-Ubi-F aaaaggatccagtgcagcgtgacccggtc BamH I
Ubi promoter r P-Ubi-R aaaacccgggcagaagtaacaccaaacaacagg Sma I
OsMT2b RNAi 1 R-MT-1 aaaagaattcgctgctccatccaacaagg EcoR I
OsMT2b RNAi 2 R-MT-2 aaaagatatcgaagcctggcacgcatgagg EcoR V
OsMT2b RNAi 3 R-MT-3 aaaaactagtgaagcctggcacgcatgagg Spe I
OsSRT1 RNAi 1 R-SRT-1 aaaagtcgacggctgttcgagctcttccattg Sal I
OsSRT1 RNAi 2 R-SRT-2 aaaaggatccataccatcaagccccacaaccag BamH I
OsSRT1 RNAi 3 R-SRT-3 aaaaaagcttcataccatcaagccccacaaccag Hind III
OsPDK1 sense f S-PDK1-F. aaaagtcgactgggtctccatatatgttcac Sal I
OsPDK1 sense r S-PDK1-R aaaaaagcttggactcattccgcgacttac Hind III
OsPDK2 sense f S-PDK2-R aaaagtcgacgccaggctctgggtcag Sal I
OsPDK1 sense r S-PDK2-R aaaaaagcttcgggtcgcgccccacg Hind III