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. Author manuscript; available in PMC: 2011 Mar 4.
Published in final edited form as: Cell Death Differ. 2010 Apr 30;17(11):1707–1716. doi: 10.1038/cdd.2010.42

Figure 1.

Figure 1

VacA-induced cell death requires proapoptotic proteins BAX and BAK. (a) Wt MEF cells were incubated with H. pylori CS, either wt or P9A mutant. After 6, 18, 24, 48 and 72 h, the Annexin-V assay was performed following the manufacturer’s instructions. Viability was determined cytofluorimetrically as the percentage of Annexin-V-Alexa 568-negative cells. (b) Bax/Bak DKO MEF cells were incubated and assayed for viability as above. Cells not exposed to H. pylori CS are defined as untreated cells. Data represent mean±S.D. of 20 independent experiments. Significance was determined using Student’s t-test for paired data of CS-intoxicated cells versus untreated cells; ***P<0.01