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. Author manuscript; available in PMC: 2011 Mar 4.
Published in final edited form as: Cell Death Differ. 2010 Apr 30;17(11):1707–1716. doi: 10.1038/cdd.2010.42

Figure 8.

Figure 8

(a) VacA G14A does not induce endosomal hijack on mitochondria. Mitochondria were purified from cells intoxicated for 24 h with H. pylori CS (either wt or G14A mutant). Using SDS-PAGE, 15 μg of proteins were then separated and membranes were probed with polyclonal anti-EEA1, anti-LAMP1 and anti-BAX antibodies. COX II was used as marker of equal loading for mitochondria. (b) Distribution of COX II in endosomal and mitochondrial fractions purified from intoxicated wt MEFs. A total of 15 μg of early endosome (EE), late endosome (LE) and mitochondria (Mt) proteins purified from wt MEF cells untreated, treated with wt H. pylori CS, or with the mutant P9A one, were loaded in SDS-PAGE and immunoblotted. The exclusive localization of COX II in mitochondria permits to exclude any contamination of endosomes by fragment of mitochondria during the subcellular fractionation