Fig. 5.

Inhibition of basal and TSAb-induced up-regulation of the expression of TPO mRNA by 1 in primary cultures of human thyrocytes. Thyrocytes were incubated in DMEM containing 2% FBS without (control) or with 30 μm 1 without (basal) or with 1:10 dilutions of GD sera at 37 C. After 48 h, the buffers were aspirated, the cells were lysed, and the levels of TPO mRNA were measured and normalized to GAPDH mRNA. The mRNA levels are presented as fold of basal levels (control) (A) and as percent inhibition = 100 − (100 × samples exposed to 1/control) (B). The data from two independent experiments with duplicate samples are shown.