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Cultured primary ESC from controls (left) and leiomyomatous uteri (right) were treated with TGF-β3. TGF-β3 treatment reduced BMPR gene expression. Untreated control ESC expressed readily detectable levels of all BMPRs. Reductions in BMPR-1A and BMPR-2 are also seen after TGF-β3 treatment. Both BMPR-1B and BMPR-2 repression occurred at lower doses of TGF-β3 in leiomyoma-associated ESC compared with control ESC. *, P < 0.0005.
