Table 3.
PCA for changes in metabolic intermediates with CR
| Components | Component loadings | Eigen value | Cumulative variance |
|---|---|---|---|
| FA change factor 1 | |||
| Linoleic acid | 0.98 | 6.54 | 0.82 |
| Oleic acid | 0.97 | ||
| Myristic acid | 0.96 | ||
| Palmitoleic acid | 0.94 | ||
| Palmitic acid | 0.93 | ||
| Stearic acid | 0.90 | ||
| α-Linolenic acid | 0.89 | ||
| Arachidonic acid | 0.60 | ||
| AA change factor 1 | |||
| Alanine | 0.84 | 6.1 | 0.40 |
| Proline | 0.83 | ||
| Methionine | 0.82 | ||
| Tyrosine | 0.77 | ||
| Histidine | 0.75 | ||
| Arginine | 0.73 | ||
| Leucine/isoleucine | 0.67 | ||
| Valine | 0.65 | ||
| Glycine | 0.64 | ||
| Phenylalanine | 0.56 | ||
| Citrulline | 0.52 | ||
| AA change factor 2 | |||
| Glutamine/glutamate | 0.87 | 2.0 | 0.54 |
| Aspartate/asparagine | 0.76 | ||
| Ornithine | 0.60 | ||
| Phenylalanine | 0.56 | ||
| AC change factor 1 | |||
| C2 | 0.70 | 10.7 | 0.24 |
| Ci4-DC/C4-DC | 0.51 | ||
| C8 | 0.74 | ||
| C6-DC | 0.59 | ||
| C10:2 | 0.63 | ||
| C10:1 | 0.71 | ||
| C10 | 0.69 | ||
| C10-OH/C8-DC | 0.57 | ||
| C12:1 | 0.84 | ||
| C12 | 0.86 | ||
| C12-OH/C10-DC | 0.45 | ||
| C14:2 | 0.73 | ||
| C14:1 | 0.84 | ||
| C14 | 0.47 | ||
| C14:1-OH | 0.48 | ||
| C14-OH/C12-DC | 0.45 | ||
| C16 | 0.68 | ||
| C18:2 | 0.63 | ||
| C18:1 | 0.66 | ||
| C16:2 | 0.57 | ||
| C16:1 | 0.64 |
Using slopes of change for each metabolite, PCA was performed separately for each metabolite class: FAs, ACs, and AAs. Key metabolites within each component (i.e. metabolites with component load ≥0.5) are presented.