Table 1. Genes identified in the RNAi screen that caused the most severe and/or penetrant tail tip morphogenesis defects.
| Gene | Sequence name | Gene function/domain | Closest human ortholog? | RNAi phenotype1 | Mutant alleles2 |
| abcx-1 | C56E6.1 | ABC-transporter | ABCG5 | Lep | - 3 |
| arl-1 | F54C9.10 | ADP-Ribosylation Factor | ARL1 | Lep | - 3 |
| blmp-1 | F25D7.3 | Zinc finger and SET domain containing protein | BLIMP1 | Ore & Lep | tm548 |
| bub-1 | R06C7.8 | Serine/threonine kinase | BUB1 | Ore & Lep | - 3 |
| cdc-42 | R07G3.1 | Rho-GTPase | CDC42 | Lep | - 3 |
| cdt-1 7 | Y54E10A.15 | DNA-replication licensing factor | CDT1 | Ore & Lep | - 3 |
| daf-4 | C05D2.1 | TGF-β Receptor | ACVR2B | Lep | - 3 |
| egl-18 | F55A8.1 | GATA Transcription factor | GATA1 | Lep | ok290 |
| inx-12 | ZK770.3 | Innexin | - 4 | Lep | - 3 |
| inx-13 | Y8G1A.2 | Innexin | - 4 | Lep | - 3 |
| mix-1 | M106.1 | Chromosome condensation complex, condensin | SMC2 | Ore | - 3 |
| nhr-25 | F11C1.6 | Nuclear hormone receptor | NR5A2 | Lep | ku217 |
| nmy-2 | F20G4.3 | Non-muscle myosin | MYH11 | Lep | - 3 |
| nob-1 | Y75B8A.2 | Abd-B Homeodomain transcription factor | HOXD12 | Lep | - 3 |
| npp-3 | K12D12.2 | Nucleoporin | NUP205 | Ore | - 3 |
| npp-6 7 | F56A3.3 | Nucleoporin | NUP160 | Ore | - 3 |
| php-3 | Y75B8A.1 | Abd-B Homeodomain transcription factor | HOXA11 | Lep | ok919 |
| plk-1 | C14B9.4 | polo-like serine/threonine kinase | PLK1 | Ore & Lep | - 3 |
| pri-2 7 | W02D9.1 | Eukaryotic-type DNA primase | PRIM2A | Ore | - 3 |
| ptl-1 | F42G9.9 | Tau-like microtubule binding protein | MAP2;MAP4 | Lep | ok621 |
| ran-3 | C26D10.1 | Nuclear export/import, RCC1 domain | HERC2;RCC1 | Ore | - 3 |
| rcn-1 | F54E7.7 | Calcipressin, negative regulator of calcineurin | RCAN1/DSCR1 | Lep | tm2021 5 |
| rme-8 | F18C12.2 | Receptor-mediated endocytosis-defective | DNAJC13 | Lep | b1023 |
| rpa-1 | F18A1.5 | DNA-binding replication protein | RPA1 | Ore | - 3 |
| sma-3 | R13F6.9 | SMAD | SMAD5 | Lep | e491 |
| smc-4 | F35G12.8 | Chromosome condensation complex, condensin | SMC4 | Ore & Lep | - 3 |
| wht-5 | F19B6.4 | ABC-transporter | ABCG1 | Lep | ok806 6 |
| xpo-2 | Y48G1A.5 | Nuclear export factor | CSE1L | Ore & Lep | - 3 |
The CGC (Caenorhabditis Genetics Center) gene name, the cosmid-based "GenePairs" name, a brief description and/or predicted human ortholog, and the tail tip phenotype produced by RNAi via feeding are provided for each gene. For some genes, mutants were analyzed and the phenotypes observed in the screen were validated.
1For phenotypes see text.
2Tested mutant alleles that show tail tip phenotypes similar to the RNAi phenotype (see Table S2 for details).
3Not tested or the mutant was not available.
4INX-12 and INX-13 are innexins, invertebrate-specific gap junction proteins, apparently unrelated to vertebrate connexins.
5Mutants were not crossed to the him-5(-) background and thus Lep phenotypes were not quantified. However, a few males (N = 2) were observed with tail tip, ray, and spicule defects.
6Mutants did not show Lep phenotypes but did display more widespread male tail defects (i.e. truncated posteriors, missing rays and fan).
7Identified as a let-7 suppressor by Ding et al. (2008) [44].