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. 2011 Mar 3;7(3):e1001320. doi: 10.1371/journal.pgen.1001320

Figure 1. STUbL mutant tdp1 Δ cells are synergistically sensitive to CPT and have increased spontaneous Top1cc levels.

Figure 1

(A) Serial dilutions of the indicated strains were spotted onto media with or without the indicated drugs. (B) ChIP-qPCR assays of an nmt41-inducible Top1-FLAG in the indicated strains, at the subtelomeres of Chr 2 (telo2R), the centromeric inner repeats of Chr 2 (cnt2), the rDNA (rDNA2), and upstream of mes1 on Chr 1. The data represents the average DNA recovery compared to the input DNA samples with standard deviations from four independent experiments. ChIP-qPCR data of nmt41-Top1-FLAG slx8-1 tdp1Δ cells grown in repressing media (+B1) acts as a negative control. Cells were grown at 25°C.