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. 2011 Mar 3;7(3):e1001320. doi: 10.1371/journal.pgen.1001320

Figure 5. Tdp1-deficient cells depend on Swi10, but not on homologous recombination repair factors, to prevent Top1-induced cell death.

Figure 5

(A) A representative tetrad dissection is shown of a cross between swi10Δ and the top1Δ tdp1Δ double mutant. The key depicts the genotypes present, which are denoted by various shapes placed around each colony. Wildtype cells do not have a shape placed around them. (B) Serial dilutions of the indicated strains expressing Top1 under a thiamine repressible promoter were spotted onto control or CPT containing media with (+B1) or without (-B1) thiamine to repress or induce Top1 expression, respectively. (C) Five fold serial dilutions of the indicated strains were spotted onto rich media that contained either no drug (YES), or camptothecin (CPT). Plates were incubated at 32°C.