Table 2. Transfection of phage DNA isolated from sewage in E. coli WG5 and C600 strains.
Sample number | ||||
Sewage 6 | Sewage 7 | Sewage 15 | ||
µg of phage DNA transfected | 2.60 | 1.14 | 1.74 | |
Ampicillin/Nal WG5 | N° of ap/nal resistant clonesa | 552 | 422 | 310 |
% bla TEM b | 10.0 | 13.6 | 16.6 | |
%bla CTX-M b | 6.8 | 1.7 | 13.3 | |
Ampicillin/Nal C600nalR | N° of ap/nal resistant clonesa | 89 | 101 | 42 |
% bla TEM | 3.6 | 7.7 | 6.2 | |
%bla CTX-M | 0 | 1.2 | 0 |
Averaged number of colonies per plate after transduction.
Percentage of colonies where these genes have been detected by PCR and confirmed by sequencing.