Table 3. Oligonucleotides used in this study.

Target gene	PCR	Sequence	Conditions	Amplimer (bp)	Reference
16SrDNA	UP	AAGAGTTTGATCCTGGCTCAG	95°C 5 min (1 cycle); 95°C 1 min, 42°C 0.5 min, and 72°C 2 min (35 cycles), 72°C 2 min (1 cycle).	1503	[61]
	LP	TACGGCTACCTTGTTACGACTT
TEM PCR	UP	CTCACCCAGAAACGCTGGTG	95°C 5 min (1 cycle). 94°C, 15 s, 63°C 1 min, 72°C, 1.3 min (30 cycles). 72°C, 4 min (1 cycle).	569	This study
	LP	ATCCGCCTCCATCCAGTCTA
TEM qPCR	UP	CACTATTCTCAGAATGACTTGGT	50°C 2 min (1 cycle). 95°C 15 min (1 cycle) 94°C for 15 s and 60°C 1 min (45 cycles).	85	[36]
	LP	TGCATAATTCTCTTACTGTCATG
	Probe	6FAM-CCAGTCACAGAAAAGCATCTTACGG-MGBNFQ
CTX-M-1 PCR	UP	ACGTTAAACACCGCCATTCC	95°C 5 min (1 cycle). 94°C, 15 s, 60°C 1 min, 72°C, 1.3 min (30 cycles) 72°C, 4 min (1 cycle).	356	This study
	LP	TCGGTGACGATTTTAGCCGC
CTX-M-1 qPCR	UP CTX-M	ACCAACGATATCGCGGTGAT	50°C 2 min (1 cycle). 95°C 15 min (1 cycle) 94°C for 15 s and 60°C 1 min (45 cycles).	101	This study
	LP CTX-M	ACATCGCGACGGCTTTCT
	Probe	6FAM – TCGTGCGCCGCTG- MGBNFQ
MecA PCR	UP	ATACTTAGTTCTTTAGCGAT	95°C 5 min (1 cycle). 94°C, 15 s; 48°C 1 min, 72°C, 1.3 min (30 cycles). 72°C, 4 min (1 cycle).	434	This study
	LP	GATAGCAGTTATATTTCTA
MecA qPCR	UP	CGCAACGTTCAATTTAATTTTGTTAA	50°C 2 min (1 cycle). 95°C 10 min (1 cycle). 95°C for 15 s and 60°C 1 min (40 cycles)	92	[38]
	LP	TGGTCTTTCTGCATTCCTGGA
	Probe	FAM-AATGACGCTATGATCCCAATCTAACTTCCACA-TAMRA